yd digestion buffer (Zymo Research)

Zymo Research is a verified supplier

Zymo Research manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Zymo Research yd digestion buffer
Yd Digestion Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yd+digestion+buffer/YD+Digestion+Buffer/10__53603_slash_actamanil__73__2025__hsef6237-63-8-16
Average 94 stars, based on 6 article reviews

yd digestion buffer - by Bioz Stars, 2026-09

94/100 stars

Images

Incubation:

Article Title: Development of Candida-Specific Real-Time PCR Assays for the Detection and Identification of Eight Medically Important Candida Species
Article Snippet: .. The cell pellet was then suspended in YD Digestion Buffer with 25 U of R-Zymolyase (Zymo Research) and incubated at 37°C for one hour. .. After vortexing thoroughly, the genomic DNA was extracted using ZR Fungal/Bacterial DNA Kit (Zymo Research) according to the manufacturer’s protocol.

Article Title: Molecular Cloning and Recombinant Protein Expression of Hypoallergen Der p 2/1S in Saccharomyces boulardii
Article Snippet: .. Cell lysis was performed using 120 μL of YD Digestion Buffer and 5 μL of Zymolyase (ZymoResearch) and incubated for 1 hour at 37°C, then followed by the addition of YD Lysis Buffer and 1 μL of 100 mM of phenylmethylsulfonyl fluoride (PMSF). .. The lysates were blotted onto a nitrocellulose membrane (Solarbio, Inc.) in duplicates.

Article Title: Development of Candida -Specific Real-Time PCR Assays for the Detection and Identification of Eight Medically Important Candida Species
Article Snippet: .. The cell pellet was then suspended in YD Digestion Buffer with 25 U of R-Zymolyase (Zymo Research) and incubated at 37°C for one hour. .. After vortexing thoroughly, the genomic DNA was extracted using ZR Fungal/Bacterial DNA Kit (Zymo Research) according to the manufacturer’s protocol.

Sterility:

Article Title: Identification of the Target of the Retrograde Response that Mediates Replicative Lifespan Extension in Saccharomyces cerevisiae
Article Snippet: .. The cells were washed with sterile, deionized water, and the pellets were resuspended in 240 μl of YD Digestion buffer (Zymo Research). .. To the suspension, 10 μl of R-Zymolyase (Zymo Research) were added, and the suspension was incubated at 37° for 1 hr.

DNA Extraction:

Article Title: Massively parallel identification of sequence motifs triggering ribosome-associated mRNA quality control.
Article Snippet: .. For genomic DNA extraction, between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11- 323), following the manufacturer’s instructions, with 240 μl YD digestion buffer and 10 μl R-Zymolyase per pellet. .. Extracted genomic DNA was sheared for 10 min (30 s on, 30 s off, on ‘High’ setting) on ice using a Diagenode Bioruptor.

Article Title: Massively parallel identification of sequence motifs triggering ribosome-associated mRNA quality control
Article Snippet: .. For genomic DNA extraction, between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions, with 240μL YD digestion buffer and 10μL R-Zymolyase per pellet. .. Extracted genomic DNA was sheared for 10 minutes (30 seconds on, 30 seconds off, on “High” setting) on ice using a Diagenode Bioruptor.

Lysis:

other:

Article Title: Artificial Repeat-Structured siRNA Precursors as Tunable Regulators for Saccharomyces cerevisiae.
Article Snippet: #+" RNA interference (RNAi) is widely used as a research tool for studying #!" biological systems and implementing artificial genetic circuits that function ##" by modulating RNA concentrations.. Here we engineered Saccharomyces #$" cerevisiae containing a heterologous Saccharomyces castelli RNAi system #%" as a test-bed for RNAi-based circuits.. Unlike prior approaches, we describe #&" a strategy that leverages repeat-structured siRNA precursors with #'" incrementally sized stems formed from 23bp-repeats to achieve modular #(" RNAi-based gene regulation.

Review